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human ngb orf  (OriGene)


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    OriGene human ngb orf
    Human Ngb Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ngb/pmc11880548-229-0-15?v=OriGene
    Average 93 stars, based on 2 article reviews
    human ngb orf - by Bioz Stars, 2026-07
    93/100 stars

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    Image Search Results


    Establishment of F3.NGB human neural stem cells. (A) F3.NGB cells are constructed via the transfection of F3 human neural stem cells with a plasmid vector encoding human NGB gene. (B) RT-PCR analysis of NGB mRNA and protein expression. (C) Immunocytochemical identification of NGB protein in F3 and F3.NGB cells. (D) Immunocytochemical staining of nestin to assess stemness. F3.NGB cells exhibit nestin expression comparable to parental F3 cells, indicating that NGB overexpression does not alter neural stem cell characteristics.

    Journal: Regenerative Therapy

    Article Title: The therapeutic potential of neuroglobin-overexpressing human neural stem cells in a photothrombosis model

    doi: 10.1016/j.reth.2025.07.013

    Figure Lengend Snippet: Establishment of F3.NGB human neural stem cells. (A) F3.NGB cells are constructed via the transfection of F3 human neural stem cells with a plasmid vector encoding human NGB gene. (B) RT-PCR analysis of NGB mRNA and protein expression. (C) Immunocytochemical identification of NGB protein in F3 and F3.NGB cells. (D) Immunocytochemical staining of nestin to assess stemness. F3.NGB cells exhibit nestin expression comparable to parental F3 cells, indicating that NGB overexpression does not alter neural stem cell characteristics.

    Article Snippet: For the overexpression of NGB ( NM_021257.4 ), cDNA clones of human NGB including HindIII (N-terminal) and Xba I (C-terminal) sites were obtained from Bioneer Corp. (Daejeon, Korea).

    Techniques: Construct, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Expressing, Staining, Over Expression